Abstract

Microdeterminationof Anti-Cancer Drug 6-Mercaptopurine in Pharmaceutical and Blood Samples by Complexationwith Os(Viii) and its Characterization by Elemental Analysis, Ftir, Nmr, Esr, Laser Raman, Tga, Dtaand Dsc Proposed Structure of Complex

Kavita Sharma Shroti

Asst. Professor 1Department of Post Graduate Studies in Chemistry 2Department of Chemistry 1Hawabagh Women’s College, Jabalpur, M.P. INDIA

ABSTRACT

Microdetermination of an anticancer drug 6-Mercaptopurine via its complexation with Os(VIII) was performed spectrophotometrically, ?max of the complex : 390 nm ?= 9252, Job's method of continuous variance and mole ratio method confirm the metal to ligand ratio as 1:1. Interference of foreign metal ions was also studied and it was found that one ppm of captopril can bear 0.0027ppm of Zn(II) 0.00049 ppm of Cu(II), 0.002 ppm of Ni(II), 0.0031ppm of Co(II), where asRu(III), Pd(II), Fe(III), interfere severely. Characterization of complex involves elemental analysis (CHN estimation), which is in good agreement with the proposed structure and confirms the metal to ligand ratio as 1:1. Spectral studies of complex include FTIR, NMR. ESR spectra, FTIR spectrum of the complex shows the binding of thiol group and carbonyl group with Os(VIII) and also the presence of co-ordinated water molecules. ESR spectrum of the complex do not exhibit any derivative peak nor any hyperfine splitting. It is therefore diamagnetic complex which propose the octahedral structure of the complex. 1HNMR studies of the complex in D2O show deprotonation of thiol group and 1 binding of sulphur as well as oxygen atom of carbonyl group to Os(VIII) hence forming the six membered chelatring. Thermal studies of the complex (TGXDT.A. and DSC) also confirm the proposed structure.

Keywords :FTIR, NMR, ESR, DSC, TGA & DTA.