Method Development and Validation of Pesticides Residues
in Rice by GC-MS/MS
Shaily Raizada* and Rahul Kumar
Department of Chemistry,
Shri Venkateshwara University, Gajraula, Amroha (Uttar Pradesh), INDIA.
ABSTRACT
A GC-MS/MS method was developed for estimation of Pesticides in Rice
using Restek Rtx-5MS column (30m X .25mm X 0.25μm) and a mobile phase of
Helium gas with gradient GC oven programming, at flow rate of 1.0 ml/min with MS
detector. The mass of Pesticides Bifenthrin and Cyfluthrin were found 226.1 and 181
respectively. The proposed method was validated for Specificity, Linearity, LOD and
LOQ, Recovery, Repeatability, Ruggedness and Matrix effect. All the parameters
were found within the acceptable limits. Linearity of Bifenthrin and Cyfluthrin was
in the range of 2.5-100μg/kg. GC-MS/MS method was specific, accurate, rugged and
suitable for the analysis of Bifenthrin and Cyfluthrin in Rice.
Keywords :Gas chromatography with mass spectrometry (GC-MS/MS), Residual
Pesticides, Sanco guideline and Method Validation.
