Method Development and Validation of Pesticides Residues in Rice by GC-MS/MS

Shaily Raizada* and Rahul Kumar

Department of Chemistry, Shri Venkateshwara University, Gajraula, Amroha (Uttar Pradesh), INDIA.


A GC-MS/MS method was developed for estimation of Pesticides in Rice using Restek Rtx-5MS column (30m X .25mm X 0.25μm) and a mobile phase of Helium gas with gradient GC oven programming, at flow rate of 1.0 ml/min with MS detector. The mass of Pesticides Bifenthrin and Cyfluthrin were found 226.1 and 181 respectively. The proposed method was validated for Specificity, Linearity, LOD and LOQ, Recovery, Repeatability, Ruggedness and Matrix effect. All the parameters were found within the acceptable limits. Linearity of Bifenthrin and Cyfluthrin was in the range of 2.5-100μg/kg. GC-MS/MS method was specific, accurate, rugged and suitable for the analysis of Bifenthrin and Cyfluthrin in Rice.

Keywords :Gas chromatography with mass spectrometry (GC-MS/MS), Residual Pesticides, Sanco guideline and Method Validation.