Abstract

Metallo-organic Copper(II) Complex in Nano Size as a New Smart Therapeutic Bomb for Hepatocellular Carcinoma

Abdou S. El-Tabl*1, Moshira M. Abd-El Wahed2, Ahmed M.Ashour1, Mohammed H. H. Abu-Setta1, Omar H.Hassanein3 and Amr Abdou Saad4

1Department of Chemistry, Faculty of Science, El-Menoufia University, Shebin El -Kom, EGYPT. 2Department of Pathology, Faculty of Medicine, El-Menoufia University, Shebin El-Kom, EGYPT. 3Department of Zoology, Faculty of Science, El-Menoufia University, Shebin El -Kom, EGYPT. 4College of Military Medicine, Cairo, EGYPT.

DOI : http://dx.doi.org/10.29055/jccs/695

ABSTRACT

Copper is a micronutrient essential to all organisms and is a critical impact in redox Chemistry, growth and development. In biology copper has a crucial role for the function of several enzymes and proteins involved energy metabolism, respiration and DNA synthesis, notably cytochrome oxidase, super dismutase, ascorbate oxidase and tyrosinase. Chemotherapeutic study of copper complex nanoparticles both in vitro and in vivo in the treatment of hepatocellular carcinoma induced in rats have been done. The treatment efficacy of copper complex nanoparticles was evaluated by measuring antioxidant activities against oxidative stress caused by diethylnitrosamine in liver tissue. The measurements included reduced glutathione content and superoxide dismutase activity, total antioxidant capacity, as well as malondialdehyde level. Liver and kidney function tests were also determined, in addition to the evaluation of serum alpha-fetoprotein in liver tissue. Histopathological examination was also performed for liver tissue. Results showed that copper nanoparticles has a high potency in the treatment of hepatocellular carcinoma which induced by diethylnitrosamine in rats as it ameliorated from the investigated parameters toward normal control animals. These findings were well appreciated with histopathological studies of diethylnitrosamine group treated with copper complex nanoparticles. It was found that Cu(II) nanoparticles can interact with antioxidant to form cancer-specific proteasome inhibitors and apoptosis inducers in liver cancer cell.

Keywords :Hepatocellular carcinoma, diethylnitrosamine, copper complex nanoparticles.