Green Synthesis, Characterization of Copper Nanoparticles Derived from Ocimum Sanctum Leaf Extract and their Antimicrobial Activities

S. Kothai*1 and Umamaheswari R2

1,2PG and Research Department of Chemistry, Ethiraj College for Women, Chennai- 600 008, INDIA.

DOI : http://dx.doi.org/10.29055/jccs/670


The present study involves the green synthesis of copper nanoparticles using Ocimum Sanctum (Tulsi) leaf extract. First, we prepared leaf extract of Ocimum Sanctum in deionised water. This extract was added to equal amount of 0.05M copper sulphate solution and the colour change from green to brown was observed which indicates the formation of Copper nanoparticles. Here, the leaf extract may act as both reducing and capping agent. The synthesized CuNps were characterized by UV-Visible, Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Analysis (EDX) and X-ray diffraction (XRD). The synthesized CuNps were in uniform Spherical and floret shape. The average particle size was found to be 29 nm. The antimicrobial study of the synthesized CuNps was established by using a gram positive, a gram negative and an opportunistic yeast pathogens.

Keywords :Ocimum Sanctum, green synthesis, CuNps, UV-Vis, FT-IR, SEM, EDX, XRD, antimicrobial.