Method Development and Validation of Spectrophotometric Determination of Azathioprine in Bulk and Tablet Form

Prafullachandra Tekale1, Smruti Tekale and A. P. Rajale3

1Department of Chemistry, G. N. Khalsa College, Matunga, Mumbai-400019, INDIA. 2Assistant Professor, Department of Applied Sciences, Vidyalankar Institute of Technology, Mumbai, INDIA. 3Associate Professor, Department of Chemistry, R.K.T. College, Ulhasnagar, INDIA.

DOI : http://dx.doi.org/10.29055/jccs/652


A simple, accurate, and precise spectrophotometric determination of Azathioprene was carried out at 285 nm. It was validated in terms of linearity, accuracy, precision, limits of detection and limits of quantification. This method proposed rapid spectrophotometric determination at room temperature. The method is simple, inexpensive and efficient for use in the analysis of bulk and pharmaceutical tablet dosage form.

Keywords :Azathioprene, spectrophotometric, validation.